› 2iza

X-ray diffraction
1.46Å resolution

APOSTREPTAVIDIN PH 2.00 I4122 STRUCTURE

Released:
DOI: 10.2210/pdb2iza/pdb
PDB entry 2iza coloured by chain, front view.
PDB entry 2iza coloured by chain, side view.
PDB entry 2iza coloured by chain, top view.
Source organism: Streptomyces avidinii
Primary publication:
Binding of biotin to streptavidin stabilizes intersubunit salt bridges between Asp61 and His87 at low pH.
Katz BA
J Mol Biol 274 776-800 (1997)
PMID: 9405158

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149694 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Streptavidin Chain: A
Molecule details ›
Chain: A
Length: 121 amino acids
Theoretical weight: 12.74 KDa
Source organism: Streptomyces avidinii
UniProt:
  • Canonical: P22629 (Residues: 37-157; Coverage: 76%)
Sequence domains: Avidin family
Structure domains: Avidin-like

Ligands and Environments

1 bound ligand:
Ligand FMT 1 x FMT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH2R
Spacegroup: I4122
Unit cell:
a: 58.07Å b: 58.07Å c: 173.38Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.21 0.21 0.22

Quick links

Citations

2 review citations

Pseudoreceptor models in drug design: bridging ligand- and receptor-based virtual screening.
Tanrikulu et al. (2008)
1 more

1 mention without citation

Improved affinity of engineered streptavidin for the Strep-tag II peptide is due to a fixed open conformation of the lid-like loop at the binding site.
Korndörfer et al. (2002)