› 2ivn

X-ray diffraction
1.65Å resolution

Function and Biology Details

Reaction catalysed: 
L-threonylcarbamoyladenylate + adenine(37) in tRNA = AMP + N(6)-L-threonylcarbamoyladenine(37) in tRNA
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-194129 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
tRNA N6-adenosine threonylcarbamoyltransferase Chain: A
Molecule details ›
Chain: A
Length: 330 amino acids
Theoretical weight: 36.24 KDa
Source organism: Pyrococcus abyssi
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9UXT7 (Residues: 1-324; Coverage: 100%)
Gene names: PAB1159, PYRAB17710, kae1
Sequence domains: tRNA N6-adenosine threonylcarbamoyltransferase
Structure domains: Nucleotidyltransferase; domain 5

Ligands and Environments

3 bound ligands:
Ligand GOL 1 x GOL
Ligand ANP 1 x ANP
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: P212121
Unit cell:
a: 61.933Å b: 69.868Å c: 75.723Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.213 0.212 0.224
Expression system: Escherichia coli

Quick links

Citations

6 review citations

The common ancestor of archaea and eukarya was not an archaeon.
Forterre P. (2013)
5 more

10 mentions without citation

The structural and functional workings of KEOPS.
Beenstock et al. (2021)
9 more