2io5 Summary

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Crystal structure of the CIA- histone H3-H4 complex

The structure was published by Natsume, R., Eitoku, M., Akai, Y., Sano, N., Horikoshi, M., and Senda, T., in 2007 in a paper entitled "Structure and function of the histone chaperone CIA/ASF1 complexed with histones H3 and H4." (abstract).

This crystal structure was determined using X-ray diffraction at a resolution of 2.7 Å and deposited in 2006.

The experimental data on which the structure is based was also deposited.

This PDB entry contains a complex of 3 biomacromolecules, namely ASF1A protein, Histone H3.1, and Histone H4.

The molecule most likely forms heterotrimers.

The following tables show cross-reference information to other databases (to obtain a list of all PDB entries sharing the same property or classification, click on the magnifying glass icon):


Chain Name UniProt Name of source organism % of UniProt sequence present in the sample Residues in the sample molecules % of residues observed
A ASF1A protein Q9Y294 (1-172) (ASF1A_HUMAN)search Homo sapienssearch < 90% 175 88%
B Histone H3.1 P84233 (2-136) (H32_XENLA)search Xenopus laevissearch 99% 135 56%
C Histone H4 P62799 (2-103) (H4_XENLA)search Xenopus laevissearch 99% 102 75%


This entry contains 3 unique UniProt proteins:

UniProt accession Name Organism PDB
Q9Y294 (1 - 172) ASF1A protein Homo sapiens
P84233 (2 - 136) Histone H3.1 Xenopus laevis
P62799 (2 - 103) Histone H4 Xenopus laevis

Chain Structural classification (SCOP) Structural classification (CATH) Sequence family (Pfam)
A ASF1-likesearch ASF1-likesearch ASF1 like histone chaperonesearch
B (P84233) Histone, subunit Asearch PF00125: Core histone H2A/H2B/H3/H4search
C (P62799) Nucleosome core histonessearch Histone, subunit Asearch PF00125: Core histone H2A/H2B/H3/H4search

Chain ID Biological process (GO) Cellular component (GO) Molecular function (GO)
A (Q9Y294) chromatin assembly or disassemblysearch nucleussearch
B (P84233) nucleosomesearch nucleoplasmsearch nucleussearch chromosomesearch DNA bindingsearch protein bindingsearch protein heterodimerization activitysearch
C (P62799) nucleosome assemblysearch chromosomesearch nucleosomesearch nucleussearch protein heterodimerization activitysearch DNA bindingsearch protein bindingsearch

Chain InterPro annotation
A Histone chaperone, ASF1-likesearch
B Histone H3/CENP-Asearch Histone coresearch Histone-foldsearch
C Histone H4search Histone coresearch Histone-foldsearch Histone H4, conserved sitesearch