› 2iey

X-ray diffraction
3.18Å resolution

Crystal Structure of mouse Rab27b bound to GDP in hexagonal space group

Released:
DOI: 10.2210/pdb2iey/pdb
PDB entry 2iey coloured by chain, front view.
PDB entry 2iey coloured by chain, side view.
PDB entry 2iey coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Structure of the small GTPase Rab27b shows an unexpected swapped dimer.
Chavas LM, Torii S, Kamikubo H, Kawasaki M, Ihara K, Kato R, Kataoka M, Izumi T, Wakatsuki S
Acta Crystallogr D Biol Crystallogr 63 769-79 (2007)
PMID: 17582168

Function and Biology Details

Reaction catalysed: 
GTP + H(2)O = GDP + phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo tetramer
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-189364 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ras-related protein Rab-27B Chains: A, B
Molecule details ›
Chains: A, B
Length: 195 amino acids
Theoretical weight: 22.5 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q99P58 (Residues: 1-193; Coverage: 89%)
Gene name: Rab27b
Sequence domains: Ras family
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

1 bound ligand:
Ligand GDP 2 x GDP
1 modified residue:
Ligand MSE 12 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-6A
Spacegroup: P6522
Unit cell:
a: 66.061Å b: 66.061Å c: 379.988Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.292 0.289 0.346
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Structure-function relationships of the G domain, a canonical switch motif.
Wittinghofer et al. (2011)
4 more

3 mentions without citation

The charge-dipole pocket: a defining feature of signaling pathway GTPase on/off switches.
Neuwald AF. (2009)
2 more