› 2idf

X-ray diffraction
2.25Å resolution

P. aeruginosa azurin N42C/M64E double mutant, BMME-linked dimer

Released:
DOI: 10.2210/pdb2idf/pdb
PDB entry 2idf coloured by chain, front view.
PDB entry 2idf coloured by chain, side view.
PDB entry 2idf coloured by chain, top view.
Source organism: Pseudomonas aeruginosa
Entry authors: Einsle O, de Jongh TE, Hoffmann M, Cavazzini D, Rossi GL, Ubbink M, Canters GW

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132373 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Azurin Chains: A, B
Molecule details ›
Chains: A, B
Length: 128 amino acids
Theoretical weight: 13.95 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P00282 (Residues: 21-148; Coverage: 100%)
Gene names: PA4922, azu
Sequence domains: Copper binding proteins, plastocyanin/azurin family
Structure domains: Cupredoxins - blue copper proteins

Ligands and Environments

4 bound ligands:
Ligand CU 2 x CU
Ligand NI 2 x NI
Ligand 144 2 x 144
Ligand OPP 1 x OPP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU300
Spacegroup: P61
Unit cell:
a: 48.004Å b: 48.004Å c: 283.399Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.219 0.219 0.261
Expression system: Escherichia coli BL21(DE3)

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