› 2id0

X-ray diffraction
2.35Å resolution

Escherichia coli RNase II

Released:
DOI: 10.2210/pdb2id0/pdb
PDB entry 2id0 coloured by chain, front view.
PDB entry 2id0 coloured by chain, side view.
PDB entry 2id0 coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Structural basis for processivity and single-strand specificity of RNase II.
Zuo Y, Vincent HA, Zhang J, Wang Y, Deutscher MP, Malhotra A
Mol Cell 24 149-56 (2006)
PMID: 16996291

Function and Biology Details

Reaction catalysed: 
Exonucleolytic cleavage in the 3'- to 5'-direction to yield nucleoside 5'-phosphates
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151826 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Exoribonuclease 2 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 644 amino acids
Theoretical weight: 73.29 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P30850 (Residues: 1-644; Coverage: 100%)
Gene names: JW1279, b1286, rnb
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand MN 4 x MN
1 modified residue:
Ligand MSE 60 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 8-BM
Spacegroup: P1
Unit cell:
a: 55.76Å b: 118.43Å c: 122.38Å
α: 107.8° β: 98.36° γ: 91.4°
R-values:
R R work R free
0.227 0.223 0.286
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

14 review citations

The critical role of RNA processing and degradation in the control of gene expression.
Arraiano et al. (2010)
13 more

3 mentions without citation

Exonuclease hDIS3L2 specifies an exosome-independent 3'-5' degradation pathway of human cytoplasmic mRNA.
Lubas et al. (2013)
2 more