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PDBe Entry: 2i9k 
Engineered Extrahelical Base Destabilization Enhances Sequence Discrimination of DNA Methyltransferase M.HhaI
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TRANSFERASE/DNA
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X-RAY DIFFRACTION
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Resolution: 2.65 Å, R-factor: 39.0%, Free R-factor: 26.2%, Spacegroup: H 3 2
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10/10/2006, deposition: 05/09/2006, last revision: 24/02/2009
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Youngblood, B.; Shieh, F.K.; De Los Rios, S.; Perona, J.J.; Reich, N.O.
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Engineered Extrahelical Base Destabilization Enhances Sequence Discrimination of DNA Methyltransferase M.HhaI
J.MOL.BIOL. vol:362, pag:334-346 (2006) [PubMed ID 16919299 ]
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Phe124Ala mutation in M.HhaI, TRANSFERASE/DNA COMPLEX
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2.1.1.37 ExPASy BRENDA (A)
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Haemophilus haemolyticus 726(A)
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Modification methylase HhaI (EC 2.1.1.37) (M.HhaI) (Cytosine-specific methyltransferase HhaI) P05102 (A)
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2hr1, 3mht
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| C, D |
5'-D(*T*GP*AP*TP*AP*GP*CP*GP*CP*TP*AP*TP*C)-3' |
DNA |
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13 |
100% |
| A |
Modification methylase HhaI |
Protein |
P05102 (MTH1_HAEPH)
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327 |
100% |
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| A |
S-ADENOSYL-L-HOMOCYSTEINE |
SAH
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