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EBI PDBe PDBeView

PDBe Entry: 2i2u view

Crystal Structure of Ribosome with messenger RNA and the Anticodon stem-loop of P-site tRNA. This file contains the 30s subunit of one 70s ribosome. The entire crystal structure contains two 70s ribosomes and is described in remark 400.
Summary
Header RIBOSOMEsearch
Method X-RAY DIFFRACTION
Experiment Resolution: 3.22 Å, R-factor: 28.7%, Free R-factor: 32.0%, Spacegroup: P 21 21 21
Released 24/10/2006, deposition: 16/08/2006, last revision: 28/04/2009
Authors Berk, V.search; Zhang, W.search; Pai, R.D.search; Cate, J.H.D.search
Primary citation Structural basis for mRNA and tRNA positioning on the ribosome.
PROC.NATL.ACAD.SCI.USAsearch vol:103, pag:15830-15834 (2006) [PubMed ID 17038497 ]search
Keywords ribosomesearch, protein-nucleic acid complexessearch
Organism Escherichia coli 562search(A B C D E F G H I J K L M N O P Q R S T U)
UniProt 30S ribosomal protein S2 P0A7V0search (B)
30S ribosomal protein S3 P0A7V3search (C)
30S ribosomal protein S4 P0A7V8search (D)
30S ribosomal protein S5 P0A7W1search (E)
30S ribosomal protein S6 [Contains: 30S ribosomal protein S6, fully modified isoform; 30S ribosomal protein S6, non-modified isoform] P02358search (F)
30S ribosomal protein S7 P02359search (G)
30S ribosomal protein S8 P0A7W7search (H)
30S ribosomal protein S9 P0A7X3search (I)
30S ribosomal protein S10 P0A7R5search (J)
30S ribosomal protein S11 P0A7R9search (K)
30S ribosomal protein S12 P0A7S3search (L)
30S ribosomal protein S13 P0A7S9search (M)
30S ribosomal protein S14 P0AG59search (N)
30S ribosomal protein S15 Q8X9M2search (O)
30S ribosomal protein S16 P0A7T3search (P)
30S ribosomal protein S17 P0AG63search (Q)
30S ribosomal protein S18 P0A7T7search (R)
30S ribosomal protein S19 P0A7U3search (S)
30S ribosomal protein S20 P0A7U7search (T)
30S ribosomal protein S21 P68679search (U)
Solvent A, I, L, N, P, T, U, X
Related entries 2i2p, 2i2t, 2i2v, 2i2p, 2i2t, 2i2u, 2i2v
Polymers
Id Name Type UniProt Residues Observed
A 16S ribosomal RNA RNA
1542 99%
W PHE TRNA (UNMODIFIED BASES) RNA
17 100%
X MRNA RNA
6 100%
B 30S ribosomal protein S2 Protein P0A7V0 (RS2_ECOLI)search
 240 90%
C 30S ribosomal protein S3 Protein P0A7V3 (RS3_ECOLI)search
 232 88%
D 30S ribosomal protein S4 Protein P0A7V8 (RS4_ECOLI)search
 205 100%
E 30S ribosomal protein S5 Protein P0A7W1 (RS5_ECOLI)search
 166 90%
F 30S ribosomal protein S6 Protein P02358 (RS6_ECOLI)search
 135 74%
G 30S ribosomal protein S7 Protein P02359 (RS7_ECOLI)search
 178 84%
H 30S ribosomal protein S8 Protein P0A7W7 (RS8_ECOLI)search
 129 100%
I 30S ribosomal protein S9 Protein P0A7X3 (RS9_ECOLI)search
 129 98%
J 30S ribosomal protein S10 Protein P0A7R5 (RS10_ECOLI)search
 103 95%
K 30S ribosomal protein S11 Protein P0A7R9 (RS11_ECOLI)search
 128 91%
L 30S ribosomal protein S12 Protein P0A7S3 (RS12_ECOLI)search
 123 100%
M 30S ribosomal protein S13 Protein P0A7S9 (RS13_ECOLI)search
 117 96%
N 30S ribosomal protein S14 Protein P0AG59 (RS14_ECOLI)search
 100 96%
O 30S ribosomal protein S15 Protein Q8X9M2 (RS15_ECO57)search
 88 100%
P 30S ribosomal protein S16 Protein P0A7T3 (RS16_ECOLI)search
 82 97%
Q 30S ribosomal protein S17 Protein P0AG63 (RS17_ECOLI)search
 83 96%
R 30S ribosomal protein S18 Protein P0A7T7 (RS18_ECOLI)search
 74 74%
S 30S ribosomal protein S19 Protein P0A7U3 (RS19_ECOLI)search
 91 86%
T 30S ribosomal protein S20 Protein P0A7U7 (RS20_ECOLI)search
 86 98%
U 30S ribosomal protein S21 Protein P68679 (RS21_ECOLI)search
 70 72%
Heterogens
Id Name Ligands
A, N, X MAGNESIUM ION MG search
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