› 2i1o

X-ray diffraction
2.4Å resolution

Crystal Structure of a Nicotinate Phosphoribosyltransferase from Thermoplasma acidophilum

Released:
DOI: 10.2210/pdb2i1o/pdb
PDB entry 2i1o coloured by chain, front view.
PDB entry 2i1o coloured by chain, side view.
PDB entry 2i1o coloured by chain, top view.
Source organism: Thermoplasma acidophilum
Entry authors: Shin DH, Kim R, Kim S-H, Berkeley Structural Genomics Center (BSGC)

Function and Biology Details

Reaction catalysed: 
Nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H(2)O = beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-190847 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Putative nicotinate phosphoribosyltransferase Chain: A
Molecule details ›
Chain: A
Length: 398 amino acids
Theoretical weight: 43.7 KDa
Source organism: Thermoplasma acidophilum
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9HJ28 (Residues: 1-392; Coverage: 100%)
Gene name: Ta1145
Sequence domains: Quinolinate phosphoribosyl transferase, C-terminal domain
Structure domains:

Ligands and Environments

5 bound ligands:
Ligand PO4 6 x PO4
Ligand ZN 2 x ZN
Ligand TRS 1 x TRS
Ligand MPD 2 x MPD
Ligand ACY 2 x ACY
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: P6322
Unit cell:
a: 135.012Å b: 135.012Å c: 120.324Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.208 0.208 0.25
Expression system: Escherichia coli

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