2hue Summary

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Structure of the H3-H4 chaperone Asf1 bound to histones H3 and H4

The structure was published by English, C.M., Adkins, M.W., Carson, J.J., Churchill, M.E., and Tyler, J.K., in 2006 in a paper entitled "Structural basis for the histone chaperone activity of asf1." (abstract).

This crystal structure was determined using X-ray diffraction at a resolution of 1.7 Å and deposited in 2006.

The experimental data on which the structure is based was also deposited.

This PDB entry contains a complex of 3 biomacromolecules, namely Anti-silencing protein 1, Histone H3, and Histone H4.

It also contains one or more heterogenic compounds (e.g., ligands, co-factors, ions, modified amino acids, etc.); see here for a complete list.

The molecule has more than one probable quaternary state observed. For more details see the quaternary structure page.

The following tables show cross-reference information to other databases (to obtain a list of all PDB entries sharing the same property or classification, click on the magnifying glass icon):


Chain Name UniProt Name of source organism % of UniProt sequence present in the sample Residues in the sample molecules % of residues observed
A Anti-silencing protein 1 P32447 (2-169) (ASF1_YEAST)search Saccharomyces cerevisiae S288csearch < 90% 175 93%
B Histone H3 P84233 (62-136) (H32_XENLA)search Xenopus laevissearch 99% 77 97%
C Histone H4 P62799 (21-103) (H4_XENLA)search Xenopus laevissearch 99% 84 97%


This entry contains 3 unique UniProt proteins:

UniProt accession Name Organism PDB
P32447 (2 - 169) Anti-silencing protein 1 Saccharomyces cerevisiae
P84233 (62 - 136) Histone H3 Xenopus laevis
P62799 (21 - 103) Histone H4 Xenopus laevis

Chain Structural classification (SCOP) Structural classification (CATH) Sequence family (Pfam)
A ASF1-likesearch ASF1-likesearch ASF1 like histone chaperonesearch
B (P84233) Histone, subunit Asearch PF00125: Core histone H2A/H2B/H3/H4search
C (P62799) Nucleosome core histonessearch Histone, subunit Asearch PF00125: Core histone H2A/H2B/H3/H4search

Chain ID Biological process (GO) Cellular component (GO) Molecular function (GO)
A (P32447) chromatin assembly or disassemblysearch nucleussearch
B (P84233) nucleosomesearch DNA bindingsearch protein heterodimerization activitysearch
C (P62799) nucleosomesearch nucleussearch DNA bindingsearch protein heterodimerization activitysearch

Chain InterPro annotation
A Histone chaperone, ASF1-likesearch
B Histone H3/CENP-Asearch Histone coresearch Histone-foldsearch
C Histone H4search Histone coresearch Histone-foldsearch