› 2h9v

X-ray diffraction
3.1Å resolution

Structural basis for induced-fit binding of Rho-kinase to the inhibitor Y27632

Released:
DOI: 10.2210/pdb2h9v/pdb
PDB entry 2h9v coloured by chain, front view.
PDB entry 2h9v coloured by chain, side view.
PDB entry 2h9v coloured by chain, top view.
Source organism: Bos taurus
Primary publication:
Structural basis for induced-fit binding of Rho-kinase to the inhibitor Y-27632.
Yamaguchi H, Miwa Y, Kasa M, Kitano K, Amano M, Kaibuchi K, Hakoshima T
J Biochem 140 305-11 (2006)
PMID: 16891330

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
homo tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-173301 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Rho-associated protein kinase 2 Chain: A
Molecule details ›
Chain: A
Length: 402 amino acids
Theoretical weight: 45.78 KDa
Source organism: Bos taurus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q28021 (Residues: 18-417; Coverage: 29%)
Gene name: ROCK2
Sequence domains: Protein kinase domain
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand Y27 1 x Y27
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL44XU
Spacegroup: P6222
Unit cell:
a: 90.781Å b: 90.781Å c: 341.279Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.234 0.232 0.277
Expression system: Spodoptera frugiperda

Quick links

Citations

6 review citations

Rho-kinase/ROCK: A key regulator of the cytoskeleton and cell polarity.
Amano et al. (2010)
5 more

6 mentions without citation

Discovery of a ROCK inhibitor, FPND, which prevents cerebral hemorrhage through maintaining vascular integrity by interference with VE-cadherin.
Li et al. (2017)
5 more