› 2h6v

X-ray diffraction
1.47Å resolution

Spectroscopic and structural study of the heterotropic linkage between halide and proton ion binding to GFP proteins- E2(GFP) APO FORM

Released:
DOI: 10.2210/pdb2h6v/pdb
PDB entry 2h6v coloured by chain, front view.
PDB entry 2h6v coloured by chain, side view.
PDB entry 2h6v coloured by chain, top view.
Source organism: Aequorea victoria
Primary publication:
Spectroscopic and structural study of proton and halide ion cooperative binding to gfp.
Arosio D, Garau G, Ricci F, Marchetti L, Bizzarri R, Nifosì R, Beltram F
Biophys J 93 232-44 (2007)
PMID: 17434942

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154537 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Green fluorescent protein Chain: A
Molecule details ›
Chain: A
Length: 242 amino acids
Theoretical weight: 27.52 KDa
Source organism: Aequorea victoria
Expression system: Escherichia coli
UniProt:
  • Canonical: P42212 (Residues: 2-238; Coverage: 100%)
Gene name: GFP
Sequence domains: Green fluorescent protein
Structure domains: Green fluorescent protein

Ligands and Environments

1 bound ligand:
Ligand NA 1 x NA
1 modified residue:
Ligand CRO 1 x CRO

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: P212121
Unit cell:
a: 51.133Å b: 62.85Å c: 69.631Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.16 0.159 0.187
Expression system: Escherichia coli

Quick links

Citations

9 review citations

Genetically encodable fluorescent biosensors for tracking signaling dynamics in living cells.
Newman et al. (2011)
8 more

1 mention without citation

Spectroscopic and structural study of proton and halide ion cooperative binding to gfp.
Arosio et al. (2007)