› 2h13

X-ray diffraction
1.58Å resolution

Crystal structure of WDR5/histone H3 complex

Released:
DOI: 10.2210/pdb2h13/pdb
PDB entry 2h13 coloured by chain, front view.
PDB entry 2h13 coloured by chain, side view.
PDB entry 2h13 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Molecular recognition of histone H3 by the WD40 protein WDR5.
Couture JF, Collazo E, Trievel RC
Nat Struct Mol Biol 13 698-703 (2006)
PMID: 16829960

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-158478 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
WD repeat-containing protein 5 Chain: A
Molecule details ›
Chain: A
Length: 317 amino acids
Theoretical weight: 34.71 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P61964 (Residues: 22-334; Coverage: 94%)
Gene names: BIG3, WDR5
Sequence domains: WD domain, G-beta repeat
Structure domains: YVTN repeat-like/Quinoprotein amine dehydrogenase
histone H3 lys-4 dimethylated Chain: B
Molecule details ›
Chain: B
Length: 10 amino acids
Theoretical weight: 1.18 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MLY 1 x MLY

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM30A
Spacegroup: C2221
Unit cell:
a: 78.68Å b: 98.86Å c: 80.58Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.177 0.166 0.2
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

50 review citations

Chromatin modifications and their function.
Kouzarides T. (2007)
49 more

8 mentions without citation

WDR5 interacts with mixed lineage leukemia (MLL) protein via the histone H3-binding pocket.
Song et al. (2008)
7 more