› 2h12

X-ray diffraction
1.85Å resolution

Structure of Acetobacter aceti citrate synthase complexed with oxaloacetate and carboxymethyldethia coenzyme A (CMX)

Released:
DOI: 10.2210/pdb2h12/pdb
PDB entry 2h12 coloured by chain, front view.
PDB entry 2h12 coloured by chain, side view.
PDB entry 2h12 coloured by chain, top view.
Source organism: Acetobacter aceti
Primary publication:
Structure of a NADH-insensitive hexameric citrate synthase that resists acid inactivation.
Francois JA, Starks CM, Sivanuntakorn S, Jiang H, Ransome AE, Nam JW, Constantine CZ, Kappock TJ
Biochemistry 45 13487-99 (2006)
PMID: 17087502

Function and Biology Details

Reaction catalysed: 
Acetyl-CoA + H(2)O + oxaloacetate = citrate + CoA
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149083 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Citrate synthase Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 436 amino acids
Theoretical weight: 48.18 KDa
Source organism: Acetobacter aceti
Expression system: Escherichia coli
UniProt:
  • Canonical: P20901 (Residues: 1-436; Coverage: 100%)
Gene name: aarA
Sequence domains: Citrate synthase, C-terminal domain
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand OAA 6 x OAA
Ligand SO4 41 x SO4
Ligand CMX 6 x CMX
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 4.2.2
Spacegroup: P212121
Unit cell:
a: 170.66Å b: 125.69Å c: 150.59Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.195 0.195 0.231
Expression system: Escherichia coli

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Citations

6 review citations

Biotechnological applications of acetic acid bacteria.
Raspor et al. (2008)
5 more

24 mentions without citation

Assembly, structure, and function of the 26S proteasome.
Bedford et al. (2010)
23 more