› 2gzf

X-ray diffraction
1.75Å resolution

Crystal structure of the E9 DNase domain with a mutant immunity protein IM9 (Y54F)

Released:
DOI: 10.2210/pdb2gzf/pdb
PDB entry 2gzf coloured by chain, front view.
PDB entry 2gzf coloured by chain, side view.
PDB entry 2gzf coloured by chain, top view.
Source organism: Escherichia coli K-12
Entry authors: Santi PS, Kolade OO, Kuhlmann UC, Kleanthous C, Hemmings AM

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140796 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Colicin-E9 immunity protein Chain: A
Molecule details ›
Chain: A
Length: 86 amino acids
Theoretical weight: 9.58 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P13479 (Residues: 1-86; Coverage: 100%)
Gene names: ceiE9, imm
Sequence domains: Colicin immunity protein / pyocin immunity protein
Structure domains: Colicin E immunity protein
Colicin-E9 Chain: B
Molecule details ›
Chain: B
Length: 134 amino acids
Theoretical weight: 15.12 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P09883 (Residues: 450-582; Coverage: 23%)
Gene names: cei, col
Sequence domains: Colicin/Pyocin-S2, DNase domain
Structure domains: Colicin/pyocin, DNase domain

Ligands and Environments

2 bound ligands:
Ligand ZN 1 x ZN
Ligand PO4 1 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: P212121
Unit cell:
a: 44.232Å b: 51.929Å c: 87.674Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.188 0.186 0.222
Expression systems:
  • Escherichia coli BL21(DE3)
  • Escherichia coli

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