› 2gvj

X-ray diffraction
2.1Å resolution

Crystal Structure of Human NMPRTase in complex with FK866

Released:
DOI: 10.2210/pdb2gvj/pdb
PDB entry 2gvj coloured by chain, front view.
PDB entry 2gvj coloured by chain, side view.
PDB entry 2gvj coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Molecular basis for the inhibition of human NMPRTase, a novel target for anticancer agents.
Khan JA, Tao X, Tong L
Nat Struct Mol Biol 13 582-8 (2006)
PMID: 16783377

Function and Biology Details

Reaction catalysed: 
Nicotinamide D-ribonucleotide + diphosphate = nicotinamide + 5-phospho-alpha-D-ribose 1-diphosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155082 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nicotinamide phosphoribosyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 491 amino acids
Theoretical weight: 55.74 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P43490 (Residues: 1-491; Coverage: 100%)
Gene names: NAMPT, PBEF, PBEF1
Sequence domains:
Structure domains: Aldolase class I

Ligands and Environments

1 bound ligand:
Ligand DGB 2 x DGB
1 modified residue:
Ligand MSE 6 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: P21
Unit cell:
a: 60.78Å b: 105.896Å c: 83.427Å
α: 90° β: 96.45° γ: 90°
R-values:
R R work R free
0.25 0.247 0.298
Expression system: Escherichia coli

Quick links

Citations

33 review citations

Nampt: linking NAD biology, metabolism and cancer.
Garten et al. (2009)
32 more

17 mentions without citation

The small molecule GMX1778 is a potent inhibitor of NAD+ biosynthesis: strategy for enhanced therapy in nicotinic acid phosphoribosyltransferase 1-deficient tumors.
Watson et al. (2009)
16 more