› 2gq8

X-ray diffraction
1.7Å resolution

Structure of SYE1, an OYE homologue from S. ondeidensis, in complex with p-hydroxyacetophenone

Released:
DOI: 10.2210/pdb2gq8/pdb
PDB entry 2gq8 coloured by chain, front view.
PDB entry 2gq8 coloured by chain, side view.
PDB entry 2gq8 coloured by chain, top view.
Source organism: Shewanella oneidensis MR-1
Primary publication:
Ligand-induced conformational changes in the capping subdomain of a bacterial old yellow enzyme homologue and conserved sequence fingerprints provide new insights into substrate binding.
van den Hemel D, Brigé A, Savvides SN, Van Beeumen J
J Biol Chem 281 28152-61 (2006)
PMID: 16857682

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-184367 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
NADH:flavin oxidoreductase/NADH oxidase N-terminal domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 365 amino acids
Theoretical weight: 39.63 KDa
Source organism: Shewanella oneidensis MR-1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8EEC8 (Residues: 1-365; Coverage: 100%)
Gene names: SO_2454, sye1
Sequence domains: NADH:flavin oxidoreductase / NADH oxidase family
Structure domains: Aldolase class I

Ligands and Environments

5 bound ligands:
Ligand BOG 1 x BOG
Ligand SO4 1 x SO4
Ligand AC6 1 x AC6
Ligand FMN 1 x FMN
Ligand TRS 1 x TRS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X13
Spacegroup: P212121
Unit cell:
a: 48.558Å b: 83.675Å c: 87.485Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.172 0.161 0.191
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Structure and function of SET and MYND domain-containing proteins.
Spellmon et al. (2015)
1 more