2gng Summary


Protein kinase A fivefold mutant model of Rho-kinase

The structure was published by Bonn, S., Herrero, S., Breitenlechner, C.B., et al., Engh, R.A., Gassel, M., and Bossemeyer, D., in 2006 in a paper entitled "Structural analysis of protein kinase A mutants with Rho-kinase inhibitor specificity" (abstract).

This crystal structure was determined using X-ray diffraction at a resolution of 1.87 Å and deposited in 2006.

The experimental data on which the structure is based was also deposited.

This PDB entry contains a complex of 2 biomacromolecules, namely cAMP-dependent protein kinase, alpha-catalytic subunit and cAMP-dependent protein kinase inhibitor alpha.

The molecule most likely forms heterodimers.

The following tables show cross-reference information to other databases (to obtain a list of all PDB entries sharing the same property or classification, click on the magnifying glass icon):

Chain Name UniProt Name of source organism % of UniProt sequence present in the sample Residues in the sample molecules % of residues observed
A cAMP-dependent protein kinase, alpha-catalytic subunit P00517 (2-351) (KAPCA_BOVIN)search Bos taurussearch 96% 350 97%
I cAMP-dependent protein kinase inhibitor alpha P63248 (6-25) (IPKA_MOUSE)search Mus musculussearch < 90% 20 100%

This entry contains 2 unique UniProt proteins:

UniProt accession Name Organism PDB
P00517 (2 - 351) cAMP-dependent protein kinase, alpha-catalytic subunit Bos taurus
P63248 (6 - 25) cAMP-dependent protein kinase inhibitor alpha

Chain Structural classification (CATH) Sequence family (Pfam)
A (P00517) Transferase(Phosphotransferase) domain 1search, Phosphorylase Kinase; domain 1search PF00069: Protein kinase domainsearch
I cAMP-dependent protein kinase inhibitorsearch

Chain ID Molecular function (GO) Biological process (GO) Cellular component (GO)
A (P00517) nucleotide bindingsearch protein kinase activitysearch protein serine/threonine kinase activitysearch cAMP-dependent protein kinase activitysearch protein serine/threonine/tyrosine kinase activitysearch protein bindingsearch ATP bindingsearch kinase activitysearch transferase activitysearch transferase activity, transferring phosphorus-containing groupssearch protein kinase bindingsearch ubiquitin protein ligase bindingsearch protein kinase A regulatory subunit bindingsearch mesoderm formationsearch neural tube closuresearch protein phosphorylationsearch phosphorylationsearch peptidyl-serine phosphorylationsearch peptidyl-threonine phosphorylationsearch regulation of osteoblast differentiationsearch protein autophosphorylationsearch positive regulation of protein export from nucleussearch sperm capacitationsearch regulation of synaptic transmissionsearch regulation of proteasomal protein catabolic processsearch regulation of protein processingsearch positive regulation of cell cycle arrestsearch cellular response to glucose stimulussearch cellular response to parathyroid hormone stimulussearch negative regulation of smoothened signaling pathway involved in dorsal/ventral neural tube patterningsearch regulation of tight junction assemblysearch nucleussearch cytoplasmsearch mitochondrionsearch centrosomesearch plasma membranesearch membranesearch AMP-activated protein kinase complexsearch neuromuscular junctionsearch extracellular vesicular exosomesearch sperm midpiecesearch ciliary basesearch

Chain InterPro annotation
A Protein kinase domainsearch AGC-kinase, C-terminalsearch Serine/threonine/dual specificity protein kinase, catalytic domainsearch Serine/threonine-protein kinase, active sitesearch Protein kinase-like domainsearch Protein kinase, ATP binding sitesearch
I cAMP-dependent protein kinase inhibitorsearch