› 2gmk

X-ray diffraction
1.65Å resolution

Crystal structure of onconase double mutant with spontaneously-assembled (AMP) 4 stack

Released:
DOI: 10.2210/pdb2gmk/pdb
PDB entry 2gmk coloured by chain, front view.
PDB entry 2gmk coloured by chain, side view.
PDB entry 2gmk coloured by chain, top view.
Source organism: Lithobates pipiens
Primary publication:
Structural basis for catalysis by onconase.
Lee JE, Bae E, Bingman CA, Phillips GN, Raines RT
J Mol Biol 375 165-77 (2008)
PMID: 18001769

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149514 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Protein P-30 Chain: A
Molecule details ›
Chain: A
Length: 104 amino acids
Theoretical weight: 11.8 KDa
Source organism: Lithobates pipiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P22069 (Residues: 1-104; Coverage: 100%)
Sequence domains: Pancreatic ribonuclease
Structure domains: Ribonuclease A-like domain

Ligands and Environments

1 bound ligand:
Ligand AMP 4 x AMP
1 modified residue:
Ligand PCA 1 x PCA

Experiments and Validation Details

Entry percentile scores
X-ray source: BRUKER AXS MICROSTAR
Spacegroup: P212121
Unit cell:
a: 28.994Å b: 52.112Å c: 66.142Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.168 0.165 0.217
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

Ribonucleases as potential modalities in anticancer therapy.
Ardelt et al. (2009)
3 more

2 mentions without citation

Structural basis for catalysis by onconase.
Lee et al. (2008)
1 more