› 2glu

X-ray diffraction
2.91Å resolution

The crystal structure of YcgJ protein from Bacillus subitilis

Released:
DOI: 10.2210/pdb2glu/pdb
PDB entry 2glu coloured by chain, front view.
PDB entry 2glu coloured by chain, side view.
PDB entry 2glu coloured by chain, top view.
Source organism: Bacillus subtilis
Entry authors: Burke T, Gorman J, Shapiro L, Burley SK, New York SGX Research Center for Structural Genomics (NYSGXRC)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-128295 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Uncharacterized methyltransferase YcgJ Chains: A, B
Molecule details ›
Chains: A, B
Length: 239 amino acids
Theoretical weight: 27.26 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O31474 (Residues: 27-253; Coverage: 90%)
Gene names: BSU03160, ycgJ
Sequence domains: Methyltransferase domain
Structure domains: Vaccinia Virus protein VP39

Ligands and Environments

2 bound ligands:
Ligand SO4 11 x SO4
Ligand SAM 2 x SAM
1 modified residue:
Ligand MSE 8 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: C2221
Unit cell:
a: 69.899Å b: 73.639Å c: 245.742Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.197 0.192 0.283
Expression system: Escherichia coli BL21(DE3)

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