› 2gjz

X-ray diffraction
2.65Å resolution

Structure of Catalytic Elimination Antibody 13G5 from a crystal in space group P2(1)

Released:
DOI: 10.2210/pdb2gjz/pdb
PDB entry 2gjz coloured by chain, front view.
PDB entry 2gjz coloured by chain, side view.
PDB entry 2gjz coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Bifunctional catalysis of proton transfer at an antibody active site.
Müller R, Debler EW, Steinmann M, Seebeck FP, Wilson IA, Hilvert D
J Am Chem Soc 129 460-1 (2007)
PMID: 17226987

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero dimer (preferred)
hetero tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-212336 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Ig-like domain-containing protein Chains: A, L
Molecule details ›
Chains: A, L
Length: 217 amino acids
Theoretical weight: 23.94 KDa
Source organism: Mus musculus
UniProt:
  • Canonical: Q65ZC0 (Residues: 1-216; Coverage: 99%)
Sequence domains:
Structure domains: Immunoglobulins
Catalytic elimination antibody 13G5 heavy chain Chains: B, H
Molecule details ›
Chains: B, H
Length: 221 amino acids
Theoretical weight: 23.48 KDa
Source organism: Mus musculus
Structure domains: Immunoglobulins

Ligands and Environments

1 bound ligand:
Ligand ZN 11 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL11-1
Spacegroup: P21
Unit cell:
a: 50.209Å b: 73.495Å c: 130.475Å
α: 90° β: 96.33° γ: 90°
R-values:
R R work R free
0.215 0.213 0.25

Quick links

Citations

2 review citations

Design of protein catalysts.
Hilvert D. (2013)
1 more

2 mentions without citation

An aspartate and a water molecule mediate efficient acid-base catalysis in a tailored antibody pocket.
Debler et al. (2009)
1 more