› 2gig

X-ray diffraction
1.83Å resolution

Alteration of sequence specificity of the type II restriction endonuclease HINCII through an indirect readout mechanism

Released:
DOI: 10.2210/pdb2gig/pdb
PDB entry 2gig coloured by chain, front view.
PDB entry 2gig coloured by chain, side view.
PDB entry 2gig coloured by chain, top view.
Source organism: Haemophilus influenzae
Primary publication:
Alteration of sequence specificity of the type II restriction endonuclease HincII through an indirect readout mechanism.
Joshi HK, Etzkorn C, Chatwell L, Bitinaite J, Horton NC
J Biol Chem 281 23852-69 (2006)
PMID: 16675462

Function and Biology Details

Reaction catalysed: 
Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-115297 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Type II restriction enzyme HincII Chains: A, B
Molecule details ›
Chains: A, B
Length: 257 amino acids
Theoretical weight: 29.84 KDa
Source organism: Haemophilus influenzae
Expression system: Escherichia coli
UniProt:
  • Canonical: P17743 (Residues: 2-258; Coverage: 100%)
Gene name: hincIIR
Sequence domains: Restriction endonuclease HincII
Structure domains: DNA mismatch repair MutH/Restriction endonuclease, type II
5'-D(*GP*CP*CP*GP*GP*TP*CP*GP*AP*CP*CP*GP*GP*C)-3' Chains: E, F
Molecule details ›
Chains: E, F
Length: 14 nucleotides
Theoretical weight: 4.28 KDa
Source organism: Haemophilus influenzae
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand NA 2 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: P21
Unit cell:
a: 50.42Å b: 90.99Å c: 67.33Å
α: 90° β: 104.1° γ: 90°
R-values:
R R work R free
0.177 0.177 0.22
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

1 review citation

Restriction endonucleases: natural and directed evolution.
Gupta et al. (2012)
17 other articles

2 mentions without citation

The crystal structure of D212 from sulfolobus spindle-shaped virus ragged hills reveals a new member of the PD-(D/E)XK nuclease superfamily.
Menon et al. (2010)
1 more