2gie Summary

pdbe.org/2gie
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HincII bound to cognate DNA GTTAAC

The structure was published by Joshi, H.K., Etzkorn, C., Chatwell, L., Bitinaite, J., and Horton, N.C., in 2006 in a paper entitled "Alteration of Sequence Specificity of the Type II Restriction Endonuclease HincII through an Indirect Readout Mechanism." (abstract).

This crystal structure was determined using X-ray diffraction at a resolution of 2.6 Å and deposited in 2006.

The experimental data on which the structure is based was also deposited.

This PDB entry contains a complex of 2 biomacromolecules, namely 5'-D(*GP*CP*CP*GP*GP*TP*TP*AP*AP*CP*CP*GP*G)-3' and Type II restriction enzyme HincII.

It also contains one or more heterogenic compounds (e.g., ligands, co-factors, ions, modified amino acids, etc.); see here for a complete list.

The molecule has more than one probable quaternary state observed. For more details see the quaternary structure page.

The following tables show cross-reference information to other databases (to obtain a list of all PDB entries sharing the same property or classification, click on the magnifying glass icon):


Chain Name UniProt Name of source organism % of UniProt sequence present in the sample Residues in the sample molecules % of residues observed
A Type II restriction enzyme HincII P17743 (2-258) (T2C2_HAEIF)search Haemophilus influenzaesearch 100% 257 100%
B Type II restriction enzyme HincII P17743 (2-258) (T2C2_HAEIF)search Haemophilus influenzaesearch 100% 257 100%
C Type II restriction enzyme HincII P17743 (2-258) (T2C2_HAEIF)search Haemophilus influenzaesearch 100% 257 100%
D Type II restriction enzyme HincII P17743 (2-258) (T2C2_HAEIF)search Haemophilus influenzaesearch 100% 257 100%


This entry contains 1 unique UniProt protein:

UniProt accession Name Organism PDB
P17743 (2 - 258) Type II restriction enzyme HincII Haemophilus influenzae

Chain Structural classification (SCOP) Structural classification (CATH) Sequence family (Pfam)
A, B, C, D (P17743) Restriction endonuclease HincIIsearch ECO RV Endonuclease; Chain Asearch PF09226: Restriction endonuclease HincIIsearch

Chain ID Biological process (GO) Molecular function (GO)
A, B, C, D (P17743) DNA catabolic process, endonucleolyticsearch DNA restriction-modification systemsearch nucleic acid phosphodiester bond hydrolysissearch endonuclease activitysearch hydrolase activitysearch DNA bindingsearch Type II site-specific deoxyribonuclease activitysearch nuclease activitysearch

Chain InterPro annotation
A, B, C, D Restriction endonuclease type II-likesearch DNA mismatch repair MutH/Restriction endonuclease, type IIsearch Restriction endonuclease, type II, HincIIsearch