› 2gh7

X-ray diffraction
1Å resolution

Epi-biotin complex with core streptavidin

Released:
DOI: 10.2210/pdb2gh7/pdb
PDB entry 2gh7 coloured by chain, front view.
PDB entry 2gh7 coloured by chain, side view.
PDB entry 2gh7 coloured by chain, top view.
Source organism: Streptomyces avidinii
Primary publication:
The high-resolution structure of (+)-epi-biotin bound to streptavidin.
Le Trong I, Aubert DG, Thomas NR, Stenkamp RE
Acta Crystallogr D Biol Crystallogr 62 576-81 (2006)
PMID: 16699183

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149694 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Streptavidin Chains: A, B
Molecule details ›
Chains: A, B
Length: 127 amino acids
Theoretical weight: 13.28 KDa
Source organism: Streptomyces avidinii
Expression system: Escherichia coli
UniProt:
  • Canonical: P22629 (Residues: 37-163; Coverage: 80%)
Sequence domains: Avidin family
Structure domains: Avidin-like

Ligands and Environments

3 bound ligands:
Ligand BTN 2 x BTN
Ligand BTQ 2 x BTQ
Ligand GOL 3 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL11-1
Spacegroup: I222
Unit cell:
a: 46.39Å b: 93.811Å c: 104.449Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.144 not available 0.152
Expression system: Escherichia coli

Quick links

Citations

4 citation in other articles

A polyhedron made of tRNAs.
Severcan et al. (2010)
3 more