› 2gat

Solution NMR

SOLUTION STRUCTURE OF THE C-TERMINAL DOMAIN OF CHICKEN GATA-1 BOUND TO DNA, NMR, REGULARIZED MEAN STRUCTURE

Released:
DOI: 10.2210/pdb2gat/pdb
PDB entry 2gat coloured by chain, front view.
PDB entry 2gat coloured by chain, side view.
PDB entry 2gat coloured by chain, top view.
Source organism: Gallus gallus
Primary publication:
Use of dipolar 1H-15N and 1H-13C couplings in the structure determination of magnetically oriented macromolecules in solution.
Tjandra N, Omichinski JG, Gronenborn AM, Clore GM, Bax A
Nat Struct Biol 4 732-8 (1997)
PMID: 9303001

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-115290 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Erythroid transcription factor Chain: A
Molecule details ›
Chain: A
Length: 66 amino acids
Theoretical weight: 7.56 KDa
Source organism: Gallus gallus
Expression system: Escherichia coli
UniProt:
  • Canonical: P17678 (Residues: 158-223; Coverage: 22%)
Gene names: ERYF1, GATA1
Sequence domains: GATA zinc finger
Structure domains: Erythroid Transcription Factor GATA-1, subunit A
DNA (5'-D(*GP*TP*TP*GP*CP*AP*GP*AP*TP*AP*AP*AP*CP*AP*TP*T)-3') Chain: B
Molecule details ›
Chain: B
Length: 16 nucleotides
Theoretical weight: 4.92 KDa
Source organism: Gallus gallus
Expression system: Not provided
DNA (5'-D(*AP*AP*TP*GP*TP*TP*TP*AP*TP*CP*TP*GP*CP*AP*AP*C)-3') Chain: C
Molecule details ›
Chain: C
Length: 16 nucleotides
Theoretical weight: 4.87 KDa
Source organism: Gallus gallus
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand ZN 1 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: simulated annealing
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

40 review citations

Theory, practice, and applications of paramagnetic relaxation enhancement for the characterization of transient low-population states of biological macromolecules and their complexes.
Clore et al. (2009)
39 more

7 mentions without citation

Structural classification of zinc fingers: survey and summary.
Krishna et al. (2003)
6 more