2g9x Summary


Structure of Thr 160 phosphorylated CDK2/cyclin A in complex with the inhibitor NU6271

The structure was published by Griffin, R.J., Henderson, A., Curtin, N.J., et al., Noble, M.E., Wang, L.Z., and Golding, B.T., in 2006 in a paper entitled "Searching for Cyclin-Dependent Kinase Inhibitors Using a New Variant of the Cope Elimination." (abstract).

This crystal structure was determined using X-ray diffraction at a resolution of 2.5 Å and deposited in 2006.

The experimental data on which the structure is based was also deposited.

This PDB entry contains a complex of 2 biomacromolecules, namely Cell division protein kinase 2 and Cyclin-A2.

It also contains one or more heterogenic compounds (e.g., ligands, co-factors, ions, modified amino acids, etc.); see here for a complete list.

The molecule has more than one probable quaternary state observed. For more details see the quaternary structure page.

The following tables show cross-reference information to other databases (to obtain a list of all PDB entries sharing the same property or classification, click on the magnifying glass icon):

Chain Name UniProt Name of source organism % of UniProt sequence present in the sample Residues in the sample molecules % of residues observed
A Cell division protein kinase 2 P24941 (1-298) (CDK2_HUMAN)search Homo sapienssearch 97% 299 100%
C Cell division protein kinase 2 P24941 (1-298) (CDK2_HUMAN)search Homo sapienssearch 97% 299 100%
B Cyclin-A2 P30274 (170-430) (CCNA2_BOVIN)search Bos taurussearch < 90% 262 100%
D Cyclin-A2 P30274 (170-430) (CCNA2_BOVIN)search Bos taurussearch < 90% 262 100%

This entry contains 2 unique UniProt proteins:

UniProt accession Name Organism PDB
P24941 (1 - 298) Cell division protein kinase 2 Homo sapiens
P30274 (170 - 430) Cyclin-A2 Bos taurus

Chain Structural classification (SCOP) Structural classification (CATH) Sequence family (Pfam)
A, C (P24941) Protein kinases, catalytic subunitsearch Phosphorylase Kinase; domain 1search, Transferase(Phosphotransferase) domain 1search PF00069: Protein kinase domainsearch
B, D Cyclinsearch Cyclin A; domain 1search Cyclin, N-terminal domainsearch, Cyclin, C-terminal domainsearch

Chain ID Molecular function (GO) Cellular component (GO) Biological process (GO)
A, C (P24941) transferase activity, transferring phosphorus-containing groupssearch ATP bindingsearch protein bindingsearch histone kinase activitysearch kinase activitysearch protein kinase activitysearch protein serine/threonine kinase activitysearch transferase activitysearch cyclin-dependent protein serine/threonine kinase activitysearch cyclin bindingsearch nucleotide bindingsearch metal ion bindingsearch nucleoplasmsearch Cajal bodysearch endosomesearch nucleussearch transcription factor complexsearch cytoplasmsearch chromosome, telomeric regionsearch cyclin-dependent protein kinase holoenzyme complexsearch cytosolsearch centrosomesearch Y chromosomesearch cytoskeletonsearch X chromosomesearch microtubule organizing centersearch condensed chromosomesearch potassium ion transportsearch mitotic nuclear divisionsearch protein phosphorylationsearch DNA repairsearch G1/S transition of mitotic cell cyclesearch DNA replicationsearch positive regulation of cell proliferationsearch G2/M transition of mitotic cell cyclesearch phosphorylationsearch meiotic nuclear divisionsearch cell cyclesearch positive regulation of transcription, DNA-templatedsearch regulation of gene silencingsearch mitotic G2 phasesearch positive regulation of DNA-dependent DNA replication initiationsearch DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrestsearch cellular response to nitric oxidesearch Ras protein signal transductionsearch regulation of ubiquitin-protein ligase activity involved in mitotic cell cyclesearch cellular response to DNA damage stimulussearch mitotic cell cyclesearch cell divisionsearch mitotic G1 DNA damage checkpointsearch anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic processsearch blood coagulationsearch histone phosphorylationsearch centrosome duplicationsearch
B, D (P30274) protein kinase bindingsearch nucleussearch regulation of cyclin-dependent protein serine/threonine kinase activitysearch regulation of cell cyclesearch regulation of G2/M transition of mitotic cell cyclesearch

Chain InterPro annotation
A, C Protein kinase domainsearch Serine/threonine/dual specificity protein kinase, catalytic domainsearch Serine/threonine-protein kinase, active sitesearch Protein kinase-like domainsearch Protein kinase, ATP binding sitesearch
B, D Cyclin, C-terminal domainsearch Cyclin, N-terminalsearch Cyclin-likesearch Cyclin A/B/D/E/Fsearch Cyclin A, chordatessearch