› 2g7f

X-ray diffraction
1.95Å resolution

The 1.95 A crystal structure of Vibrio cholerae extracellular endonuclease I

Released:
DOI: 10.2210/pdb2g7f/pdb
PDB entry 2g7f coloured by chain, front view.
PDB entry 2g7f coloured by chain, side view.
PDB entry 2g7f coloured by chain, top view.
Source organism: Vibrio cholerae
Primary publication:
The structure of Vibrio cholerae extracellular endonuclease I reveals the presence of a buried chloride ion.
Altermark B, Smalås AO, Willassen NP, Helland R
Acta Crystallogr D Biol Crystallogr 62 1387-91 (2006)
PMID: 17057343

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-174126 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Extracellular deoxyribonuclease Chain: A
Molecule details ›
Chain: A
Length: 211 amino acids
Theoretical weight: 24.53 KDa
Source organism: Vibrio cholerae
Expression system: Escherichia coli
UniProt:
  • Canonical: Q2XSK9 (Residues: 21-231; Coverage: 100%)
Gene names: ERS013165_03800, dns
Sequence domains: Endonuclease I

Ligands and Environments

2 bound ligands:
Ligand CL 1 x CL
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P212121
Unit cell:
a: 40.4Å b: 64.75Å c: 75.78Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.179 0.177 0.233
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Nucleases: diversity of structure, function and mechanism.
Yang W. (2011)
8 other articles

1 mention without citation

A comparative study of cold- and warm-adapted Endonucleases A using sequence analyses and molecular dynamics simulations.
Michetti et al. (2017)