› 2fwq

X-ray diffraction
1.4Å resolution

Reduced enolate chromophore intermediate for Y66H GFP variant

Released:
DOI: 10.2210/pdb2fwq/pdb
PDB entry 2fwq coloured by chain, front view.
PDB entry 2fwq coloured by chain, side view.
PDB entry 2fwq coloured by chain, top view.
Source organism: Aequorea victoria
Primary publication:
Structural evidence for an enolate intermediate in GFP fluorophore biosynthesis.
Barondeau DP, Tainer JA, Getzoff ED
J Am Chem Soc 128 3166-8 (2006)
PMID: 16522096

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154537 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Green fluorescent protein Chain: A
Molecule details ›
Chain: A
Length: 237 amino acids
Theoretical weight: 26.73 KDa
Source organism: Aequorea victoria
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P42212 (Residues: 2-238; Coverage: 100%)
Gene name: GFP
Sequence domains: Green fluorescent protein
Structure domains: Green fluorescent protein

Ligands and Environments

1 bound ligand:
Ligand MG 1 x MG
1 modified residue:
Ligand XXY 1 x XXY

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL7-1
Spacegroup: P212121
Unit cell:
a: 51.135Å b: 62.451Å c: 71.453Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.136 not available 0.198
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

5 review citations

Fluorescent proteins: maturation, photochemistry and photophysics.
Remington SJ. (2006)
4 more

2 mentions without citation

Kinetic isotope effect studies on the de novo rate of chromophore formation in fast- and slow-maturing GFP variants.
Pouwels et al. (2008)
1 more