› 2fwh

X-ray diffraction
0.99Å resolution

atomic resolution crystal structure of the C-terminal domain of the electron transfer catalyst DsbD (reduced form at pH7)

Released:
DOI: 10.2210/pdb2fwh/pdb
PDB entry 2fwh coloured by chain, front view.
PDB entry 2fwh coloured by chain, side view.
PDB entry 2fwh coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
High-resolution structures of Escherichia coli cDsbD in different redox states: A combined crystallographic, biochemical and computational study.
Stirnimann CU, Rozhkova A, Grauschopf U, Böckmann RA, Glockshuber R, Capitani G, Grütter MG
J Mol Biol 358 829-45 (2006)
PMID: 16545842

Function and Biology Details

Reaction catalysed: 
Protein dithiol + NAD(P)(+) = protein disulfide + NAD(P)H
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153206 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Thiol:disulfide interchange protein DsbD Chain: A
Molecule details ›
Chain: A
Length: 134 amino acids
Theoretical weight: 15.1 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P36655 (Residues: 438-565; Coverage: 23%)
Gene names: JW5734, b4136, cutA2, cycZ, dipZ, dsbD
Sequence domains: Thioredoxin-like domain
Structure domains: Glutaredoxin

Ligands and Environments

2 bound ligands:
Ligand IOD 3 x IOD
Ligand PEG 1 x PEG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P212121
Unit cell:
a: 30.289Å b: 46.072Å c: 74.07Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.113 0.113 0.146
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Understanding the pK(a) of redox cysteines: the key role of hydrogen bonding.
Roos et al. (2013)
3 more

4 mentions without citation

The interplay between the disulfide bond formation pathway and cytochrome c maturation in Escherichia coli.
Mavridou et al. (2012)
3 more