› 2fta

X-ray diffraction
1.61Å resolution

Structure of Cu(II)azurin with the metal-binding loop sequence "CTFPGHSALM" replaced with "CTPHPFM"

Released:
DOI: 10.2210/pdb2fta/pdb
PDB entry 2fta coloured by chain, front view.
PDB entry 2fta coloured by chain, side view.
PDB entry 2fta coloured by chain, top view.
Source organism: Pseudomonas aeruginosa
Primary publication:
Basic requirements for a metal-binding site in a protein: the influence of loop shortening on the cupredoxin azurin.
Li C, Yanagisawa S, Martins BM, Messerschmidt A, Banfield MJ, Dennison C
Proc Natl Acad Sci U S A 103 7258-63 (2006)
PMID: 16651527

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132372 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Azurin Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 125 amino acids
Theoretical weight: 13.73 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli
UniProt:
  • Canonical: P00282 (Residues: 21-148; Coverage: 98%)
Gene names: PA4922, azu
Sequence domains: Copper binding proteins, plastocyanin/azurin family
Structure domains: Cupredoxins - blue copper proteins

Ligands and Environments

4 bound ligands:
Ligand CU 4 x CU
Ligand 2PE 2 x 2PE
Ligand EOH 3 x EOH
Ligand PEG 1 x PEG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ENRAF-NONIUS
Spacegroup: P21
Unit cell:
a: 36.84Å b: 97.63Å c: 61.12Å
α: 90° β: 94.31° γ: 90°
R-values:
R R work R free
0.233 0.233 0.269
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Inner- and outer-sphere metal coordination in blue copper proteins.
Warren et al. (2012)
2 more

2 mentions without citation

Basic requirements for a metal-binding site in a protein: the influence of loop shortening on the cupredoxin azurin.
Li et al. (2006)
1 more