Function and Biology Details
Reactions catalysed:
Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine
Biochemical function:
- not assigned
Biological process:
- not assigned
Cellular component:
- not assigned
Sequence domains:
Structure analysis Details
Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-169501 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Ubiquitin carboxyl-terminal hydrolase 7
Molecule details ›
Chain: A
Length: 155 amino acids
Theoretical weight: 18.13 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
Sequence domains: MATH domain
Structure domains: Apoptosis, Tumor Necrosis Factor Receptor Associated Protein 2; Chain A
Length: 155 amino acids
Theoretical weight: 18.13 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
- Canonical:
Q93009 (Residues: 54-205; Coverage: 14%)
Sequence domains: MATH domain
Structure domains: Apoptosis, Tumor Necrosis Factor Receptor Associated Protein 2; Chain A
E3 ubiquitin-protein ligase Mdm2
Molecule details ›
Chain: B
Length: 6 amino acids
Theoretical weight: 635 Da
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
Length: 6 amino acids
Theoretical weight: 635 Da
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
- Canonical: Q00987 (Residues: 145-150; Coverage: 1%)
Ligands and Environments
No bound ligands
No modified residues
Experiments and Validation Details
X-ray source:
OTHER
Spacegroup:
P41
Expression systems:
- Escherichia coli BL21(DE3)
- Not provided
