2fo5 Summary

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Crystal structure of recombinant barley cysteine endoprotease B isoform 2 (EP-B2) in complex with leupeptin

The structure was published by Bethune, M.T., Strop, P., Tang, Y., Sollid, L.M., and Khosla, C., in 2006 in a paper entitled "Heterologous Expression, Purification, Refolding, and Structural-Functional Characterization of EP-B2, a Self-Activating Barley Cysteine Endoprotease." (abstract).

This crystal structure was determined using X-ray diffraction at a resolution of 2.2 Å and deposited in 2006.

The experimental data on which the structure is based was also deposited.

This PDB entry contains a complex of 2 biomacromolecules, namely Cysteine proteinase EP-B 2 and ACE-LEU-LEU-argininal (leupeptin).

It also contains one or more heterogenic compounds (e.g., ligands, co-factors, ions, modified amino acids, etc.); see here for a complete list.

The molecule has more than one probable quaternary state observed. For more details see the quaternary structure page.

The following tables show cross-reference information to other databases (to obtain a list of all PDB entries sharing the same property or classification, click on the magnifying glass icon):


Chain Name UniProt Name of source organism % of UniProt sequence present in the sample Residues in the sample molecules % of residues observed
A Cysteine proteinase EP-B 2 P25250 (131-373) (CYSP2_HORVU)search Hordeum vulgaresearch < 90% 262 85%
B Cysteine proteinase EP-B 2 P25250 (131-373) (CYSP2_HORVU)search Hordeum vulgaresearch < 90% 262 85%
C Cysteine proteinase EP-B 2 P25250 (131-373) (CYSP2_HORVU)search Hordeum vulgaresearch < 90% 262 85%
D Cysteine proteinase EP-B 2 P25250 (131-373) (CYSP2_HORVU)search Hordeum vulgaresearch < 90% 262 85%


This entry contains 1 unique UniProt protein:

UniProt accession Name Organism PDB
P25250 (131 - 373) Cysteine proteinase EP-B 2 Hordeum vulgare

Chain Structural classification (CATH) Sequence family (Pfam)
A, B, C, D Cysteine proteinasessearch Papain family cysteine proteasesearch

Chain Molecule NORINE reference
E, F, G, HACE-LEU-LEU-argininal (leupeptin)NOR00487, NOR00487

Chain ID Biological process (GO) Molecular function (GO)
A, B, C, D (P25250) proteolysissearch cysteine-type peptidase activitysearch

Chain InterPro annotation
A, B, C, D Cysteine peptidase, cysteine active sitesearch Peptidase C1A, papain C-terminalsearch Peptidase C1Asearch Cysteine peptidase, histidine active sitesearch Cysteine peptidase, asparagine active sitesearch