2fo1

X-ray diffraction
3.12Å resolution

Crystal Structure of the CSL-Notch-Mastermind ternary complex bound to DNA

Released:
Source organism: Caenorhabditis elegans

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-115273 (preferred)
Entry contents:
3 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (5 distinct):
Suppressor of hairless protein homolog Chain: A
Molecule details ›
Chain: A
Length: 477 amino acids
Theoretical weight: 53.76 KDa
Source organism: Caenorhabditis elegans
Expression system: Escherichia coli
UniProt:
  • Canonical: V6CLJ5 (Residues: 309-780; Coverage: 60%)
Gene names: K08B4.1, lag-1
Sequence domains:
Structure domains:
Protein lag-3 Chain: D
Molecule details ›
Chain: D
Length: 85 amino acids
Theoretical weight: 9.76 KDa
Source organism: Caenorhabditis elegans
Expression system: Escherichia coli
UniProt:
  • Canonical: Q09260 (Residues: 49-132; Coverage: 17%)
Gene names: C32A3.1, lag-3, sel-8
Structure domains: Single alpha-helices involved in coiled-coils or other helix-helix interfaces
Protein lin-12 Chain: E
Molecule details ›
Chain: E
Length: 373 amino acids
Theoretical weight: 42.46 KDa
Source organism: Caenorhabditis elegans
Expression system: Escherichia coli
UniProt:
  • Canonical: P14585 (Residues: 931-1301; Coverage: 26%)
Gene names: R107.8, lin-12
Sequence domains: Ankyrin repeat
Structure domains: Ankyrin repeat-containing domain
5'-D(*TP*TP*AP*CP*TP*GP*TP*GP*GP*GP*AP*AP*AP*GP*A)-3' Chain: B
Molecule details ›
Chain: B
Length: 15 nucleotides
Theoretical weight: 4.67 KDa
5'-D(*AP*AP*TP*CP*TP*TP*TP*CP*CP*CP*AP*CP*AP*GP*T)-3' Chain: C
Molecule details ›
Chain: C
Length: 15 nucleotides
Theoretical weight: 4.5 KDa

Ligands and Environments

No bound ligands
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 17-ID
Spacegroup: P212121
Unit cell:
a: 66.093Å b: 96.785Å c: 243.538Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.273 0.273 0.34
Expression system: Escherichia coli