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Crystal Structure of the CSL-Notch-Mastermind ternary complex bound to DNA

X-ray diffraction
3.12Å resolution
PDB entry 2fo1 coloured by chain, front view.
PDB entry 2fo1 coloured by chain, side view.
PDB entry 2fo1 coloured by chain, top view.
Source organism: Caenorhabditis elegans
Assembly composition:
hetero pentamer (preferred)
Entry contents: 3 distinct polypeptide molecules
2 distinct DNA molecules

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Assemblies

Assembly 1 (preferred)
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Assembly Name: CSL-Notch-Mastermind transcription factor complex and DNA
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Multimeric state: hetero pentamer
Accessible surface area: 40040.47 Å2
Buried surface area: 12825.94 Å2
Dissociation area: 2,244.89 Å2
Dissociation energy (ΔGdiss): -1.49 kcal/mol
Dissociation entropy (TΔSdiss): 12.6 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-115273

Macromolecules

Suppressor of hairless protein homolog
Chain: A
Length: 477 amino acids
Theoretical weight: 53.76 KDa
Source organism: Caenorhabditis elegans
Expression system: Escherichia coli
UniProt:
  • Canonical: V6CLJ5 (Residues: 309-780; Coverage: 60%)
Gene names: K08B4.1, lag-1
Pfam:
InterPro:
CATH:
SCOP:
Suppressor of hairless protein homolog in PDB entry 2fo1, assembly 1, front view.
Suppressor of hairless protein homolog in PDB entry 2fo1, assembly 1, side view.
Suppressor of hairless protein homolog in PDB entry 2fo1, assembly 1, top view.
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Protein lag-3
Chain: D
Length: 85 amino acids
Theoretical weight: 9.76 KDa
Source organism: Caenorhabditis elegans
Expression system: Escherichia coli
UniProt:
  • Canonical: Q09260 (Residues: 49-132; Coverage: 17%)
Gene names: C32A3.1, lag-3, sel-8
InterPro: Transcription activator LAG-3
CATH: Single alpha-helices involved in coiled-coils or other helix-helix interfaces
SCOP: Lag-3 N-terminal region
Protein lag-3 in PDB entry 2fo1, assembly 1, front view.
Protein lag-3 in PDB entry 2fo1, assembly 1, side view.
Protein lag-3 in PDB entry 2fo1, assembly 1, top view.
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Protein lin-12
Chain: E
Length: 373 amino acids
Theoretical weight: 42.46 KDa
Source organism: Caenorhabditis elegans
Expression system: Escherichia coli
UniProt:
  • Canonical: P14585 (Residues: 931-1301; Coverage: 26%)
Gene names: R107.8, lin-12
Pfam: Ankyrin repeat
InterPro:
CATH: Ankyrin repeat-containing domain
SCOP: Ankyrin repeat
Protein lin-12 in PDB entry 2fo1, assembly 1, front view.
Protein lin-12 in PDB entry 2fo1, assembly 1, side view.
Protein lin-12 in PDB entry 2fo1, assembly 1, top view.
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DNA chain B
Name: 5'-D(*TP*TP*AP*CP*TP*GP*TP*GP*GP*GP*AP*AP*AP*GP*A)-3'
Representative chains: B
Length: 15 nucleotides
Theoretical weight: 4.67 KDa
5'-D(*TP*TP*AP*CP*TP*GP*TP*GP*GP*GP*AP*AP*AP*GP*A)-3' in PDB entry 2fo1, assembly 1, front view.
5'-D(*TP*TP*AP*CP*TP*GP*TP*GP*GP*GP*AP*AP*AP*GP*A)-3' in PDB entry 2fo1, assembly 1, side view.
5'-D(*TP*TP*AP*CP*TP*GP*TP*GP*GP*GP*AP*AP*AP*GP*A)-3' in PDB entry 2fo1, assembly 1, top view.
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DNA chain C
Name: 5'-D(*AP*AP*TP*CP*TP*TP*TP*CP*CP*CP*AP*CP*AP*GP*T)-3'
Representative chains: C
Length: 15 nucleotides
Theoretical weight: 4.5 KDa
5'-D(*AP*AP*TP*CP*TP*TP*TP*CP*CP*CP*AP*CP*AP*GP*T)-3' in PDB entry 2fo1, assembly 1, front view.
5'-D(*AP*AP*TP*CP*TP*TP*TP*CP*CP*CP*AP*CP*AP*GP*T)-3' in PDB entry 2fo1, assembly 1, side view.
5'-D(*AP*AP*TP*CP*TP*TP*TP*CP*CP*CP*AP*CP*AP*GP*T)-3' in PDB entry 2fo1, assembly 1, top view.
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