› 2fn1

X-ray diffraction
2.1Å resolution

Crystal structures of Yersinia enterocolitica salicylate synthase (Irp9) in complex with the reaction products salicylate and pyruvate

Released:
DOI: 10.2210/pdb2fn1/pdb
PDB entry 2fn1 coloured by chain, front view.
PDB entry 2fn1 coloured by chain, side view.
PDB entry 2fn1 coloured by chain, top view.
Source organism: Yersinia enterocolitica
Primary publication:
Crystal structures of Yersinia enterocolitica salicylate synthase and its complex with the reaction products salicylate and pyruvate.
Kerbarh O, Chirgadze DY, Blundell TL, Abell C
J Mol Biol 357 524-34 (2006)
PMID: 16434053

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-194965 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Chorismate-utilising enzyme C-terminal domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 437 amino acids
Theoretical weight: 48.31 KDa
Source organism: Yersinia enterocolitica
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9X9I8 (Residues: 1-434; Coverage: 100%)
Gene name: irp9
Sequence domains: chorismate binding enzyme
Structure domains: Anthranilate synthase

Ligands and Environments

3 bound ligands:
Ligand MG 2 x MG
Ligand PYR 2 x PYR
Ligand SAL 2 x SAL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH3R
Spacegroup: P21
Unit cell:
a: 56.571Å b: 145.298Å c: 58.86Å
α: 90° β: 108.39° γ: 90°
R-values:
R R work R free
0.191 0.188 0.245
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Siderophore-based iron acquisition and pathogen control.
Miethke et al. (2007)
5 more

11 mentions without citation

Breaking a pathogen's iron will: Inhibiting siderophore production as an antimicrobial strategy.
Lamb AL. (2015)
10 more