› 2fmn

X-ray diffraction
2.05Å resolution

Ala177Val mutant of E. coli Methylenetetrahydrofolate Reductase complex with LY309887

Released:
DOI: 10.2210/pdb2fmn/pdb
PDB entry 2fmn coloured by chain, front view.
PDB entry 2fmn coloured by chain, side view.
PDB entry 2fmn coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Structural perturbations in the Ala --> Val polymorphism of methylenetetrahydrofolate reductase: how binding of folates may protect against inactivation.
Pejchal R, Campbell E, Guenther BD, Lennon BW, Matthews RG, Ludwig ML
Biochemistry 45 4808-18 (2006)
PMID: 16605249

Function and Biology Details

Reaction catalysed: 
5-methyltetrahydrofolate + NAD(+) = 5,10-methylenetetrahydrofolate + NADH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142677 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
5,10-methylenetetrahydrofolate reductase Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 304 amino acids
Theoretical weight: 34.24 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0AEZ1 (Residues: 1-296; Coverage: 100%)
Gene names: JW3913, b3941, metF
Sequence domains: Methylenetetrahydrofolate reductase
Structure domains: TIM Barrel

Ligands and Environments

2 bound ligands:
Ligand FAD 3 x FAD
Ligand 4HF 3 x 4HF
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: C2
Unit cell:
a: 101.56Å b: 128.64Å c: 96.744Å
α: 90° β: 120.87° γ: 90°
R-values:
R R work R free
0.217 0.217 0.25
Expression system: Escherichia coli

Quick links

Citations

11 review citations

Biomarkers of Nutrition for Development-Folate Review.
Bailey et al. (2015)
10 more

3 mentions without citation

Structural perturbations in the Ala --> Val polymorphism of methylenetetrahydrofolate reductase: how binding of folates may protect against inactivation.
Pejchal et al. (2006)
2 more