› 2fe1

X-ray diffraction
2.2Å resolution

Crystal Structure of PAE0151 from Pyrobaculum aerophilum

Released:
DOI: 10.2210/pdb2fe1/pdb
PDB entry 2fe1 coloured by chain, front view.
PDB entry 2fe1 coloured by chain, side view.
PDB entry 2fe1 coloured by chain, top view.
Source organism: Pyrobaculum aerophilum str. IM2
Primary publication:
Crystal structure of PAE0151 from Pyrobaculum aerophilum, a PIN-domain (VapC) protein from a toxin-antitoxin operon.
Bunker RD, McKenzie JL, Baker EN, Arcus VL
Proteins 72 510-8 (2008)
PMID: 18398909

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-187437 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribonuclease VapC3 Chain: A
Molecule details ›
Chain: A
Length: 156 amino acids
Theoretical weight: 17.75 KDa
Source organism: Pyrobaculum aerophilum str. IM2
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8ZZP3 (Residues: 1-131; Coverage: 100%)
Gene names: PAE0151, vapC3
Sequence domains: PIN domain
Structure domains: 5'-nuclease

Ligands and Environments

3 bound ligands:
Ligand MN 1 x MN
Ligand CL 1 x CL
Ligand CA 1 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P6122
Unit cell:
a: 72.343Å b: 72.343Å c: 96.803Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.179 0.174 0.229
Expression system: Escherichia coli

Quick links

Citations

7 review citations

The PIN-domain ribonucleases and the prokaryotic VapBC toxin-antitoxin array.
Arcus et al. (2011)
6 more

10 mentions without citation

Wake me when it's over - Bacterial toxin-antitoxin proteins and induced dormancy.
Coussens et al. (2016)
9 more