› 2fb8

X-ray diffraction
2.9Å resolution

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-147194 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Serine/threonine-protein kinase B-raf Chains: A, B
Molecule details ›
Chains: A, B
Length: 281 amino acids
Theoretical weight: 31.91 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P15056 (Residues: 445-723; Coverage: 36%)
Gene names: BRAF, BRAF1, RAFB1
Sequence domains: Protein tyrosine and serine/threonine kinase
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand 215 2 x 215
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-1
Spacegroup: P41212
Unit cell:
a: 95.638Å b: 95.638Å c: 159.222Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.203 0.199 0.291
Expression system: Spodoptera frugiperda

Quick links

Citations

38 review citations

Hyperactive Ras in developmental disorders and cancer.
Schubbert et al. (2007)
37 more

22 mentions without citation

New perspectives for targeting RAF kinase in human cancer.
Karoulia et al. (2017)
21 more