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The structure of mitochondrial PEPCK, Complex with Mn and GDP

X-ray diffraction
2.09Å resolution
PDB entry 2fah coloured by chain, front view.
PDB entry 2fah coloured by chain, side view.
PDB entry 2fah coloured by chain, top view.
Source organism: Gallus gallus
Assembly composition:
monomeric (preferred)
Entry contents: 1 distinct polypeptide molecule

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Assemblies

Assembly 1
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Assembly Name: Phosphoenolpyruvate carboxykinase [GTP], mitochondrial
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Multimeric state: monomeric
Accessible surface area: 22293.3 Å2
Buried surface area: 1454.75 Å2
Dissociation area: 96.74 Å2
Dissociation energy (ΔGdiss): 8.1 kcal/mol
Dissociation entropy (TΔSdiss): -0.12 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-149338
Assembly 2
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Assembly Name: Phosphoenolpyruvate carboxykinase [GTP], mitochondrial
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Multimeric state: monomeric
Accessible surface area: 22397.59 Å2
Buried surface area: 1579.65 Å2
Dissociation area: 55.92 Å2
Dissociation energy (ΔGdiss): -1.36 kcal/mol
Dissociation entropy (TΔSdiss): 1.22 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-149338
Assembly 3 (preferred)
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Assembly Name: Phosphoenolpyruvate carboxykinase [GTP], mitochondrial
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Multimeric state: monomeric
Accessible surface area: 22600.1 Å2
Buried surface area: 1649.61 Å2
Dissociation area: 89.57 Å2
Dissociation energy (ΔGdiss): -3.83 kcal/mol
Dissociation entropy (TΔSdiss): 2.52 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-149338
Assembly 4
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Assembly Name: Phosphoenolpyruvate carboxykinase [GTP], mitochondrial
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Multimeric state: monomeric
Accessible surface area: 22110.89 Å2
Buried surface area: 1758.93 Å2
Dissociation area: 144.92 Å2
Dissociation energy (ΔGdiss): -3.7 kcal/mol
Dissociation entropy (TΔSdiss): 2.51 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-149338

Macromolecules

Phosphoenolpyruvate carboxykinase [GTP], mitochondrial
Chains: A, B, C, D
Length: 608 amino acids
Theoretical weight: 67.39 KDa
Source organism: Gallus gallus
UniProt:
  • Canonical: P21642 (Residues: 34-128, 130-641; Coverage: 95%)
Gene name: PCK2
Pfam:
InterPro:
CATH:
Phosphoenolpyruvate carboxykinase [GTP], mitochondrial in PDB entry 2fah, assembly 3, front view.
Phosphoenolpyruvate carboxykinase [GTP], mitochondrial in PDB entry 2fah, assembly 3, side view.
Phosphoenolpyruvate carboxykinase [GTP], mitochondrial in PDB entry 2fah, assembly 3, top view.
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