2f8x Summary

pdbe.org/2f8x
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Crystal structure of activated Notch, CSL and MAML on HES-1 promoter DNA sequence

The structure was published by Nam, Y., Sliz, P., Song, L., Aster, J.C., and Blacklow, S.C., in 2006 in a paper entitled "Structural basis for cooperativity in recruitment of MAML coactivators to Notch transcription complexes." (abstract).

This crystal structure was determined using X-ray diffraction at a resolution of 3.25 Å and deposited in 2005.

The experimental data on which the structure is based was also deposited.

This entry contains 8 copies of 5 unique biopolymers (complete list).

The molecule most likely forms heteropentamers.

The following tables show cross-reference information to other databases (to obtain a list of all PDB entries sharing the same property or classification, click on the magnifying glass icon):


Chain Name UniProt Name of source organism % of UniProt sequence present in the sample Residues in the sample molecules % of residues observed
C Recombining binding protein suppressor of hairless, isoform 4 Q06330 (23-449) (SUH_HUMAN)search Homo sapienssearch < 90% 434 97%
K Neurogenic locus notch homolog protein 1 P46531 (1872-2126) (NOTC1_HUMAN)search Homo sapienssearch < 90% 256 85%
M Mastermind-like protein 1 Q92585 (13-74) (MAML1_HUMAN)search Homo sapienssearch < 90% 63 87%


This entry contains 3 unique UniProt proteins:

UniProt accession Name Organism PDB
Q06330 (23 - 449) Recombining binding protein suppressor of hairless, isoform 4 Homo sapiens
P46531 (1872 - 2126) Neurogenic locus notch homolog protein 1 Homo sapiens
Q92585 (13 - 74) Mastermind-like protein 1 Homo sapiens

Chain Structural classification (CATH) Sequence family (Pfam)
C Lin-12 and glp-1 transcriptional regulator; domain 1search, Trefoil (Acidic Fibroblast Growth Factor, subunit A)search, Immunoglobulinssearch IPT/TIG domainsearch, Beta-trefoil DNA-binding domainsearch, LAG1, DNA bindingsearch
K Serine Threonine Protein Phosphatase 5, Tetratricopeptide repeatsearch Ankyrin repeatsearch, Ankyrin repeats (3 copies)search, Ankyrin repeats (many copies)search
M MamL-1 domainsearch

Chain ID Cellular component (GO) Molecular function (GO) Biological process (GO)
C (Q06330) nucleussearch sequence-specific DNA binding transcription factor activitysearch RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activitysearch RNA polymerase II core promoter proximal region sequence-specific DNA bindingsearch DNA bindingsearch regulation of transcription, DNA-templatedsearch
M (Q92585) nuclear specksearch transcription coactivator activitysearch Notch signaling pathwaysearch positive regulation of transcription from RNA polymerase II promotersearch

Chain InterPro annotation
C IPT domainsearch p53-like transcription factor, DNA-bindingsearch Immunoglobulin-like foldsearch Immunoglobulin E-setsearch Beta-trefoil DNA-binding domainsearch LAG1, DNA bindingsearch
K Ankyrin repeatsearch Ankyrin repeat-containing domainsearch Neurogenic locus Notch 1search
M Neurogenic mastermind-like, N-terminalsearch