› 2f8x

X-ray diffraction
3.25Å resolution

Crystal structure of activated Notch, CSL and MAML on HES-1 promoter DNA sequence

Released:
DOI: 10.2210/pdb2f8x/pdb
PDB entry 2f8x coloured by chain, front view.
PDB entry 2f8x coloured by chain, side view.
PDB entry 2f8x coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis for cooperativity in recruitment of MAML coactivators to Notch transcription complexes.
Nam Y, Sliz P, Song L, Aster JC, Blacklow SC
Cell 124 973-83 (2006)
PMID: 16530044

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-115244 (preferred)
Entry contents:
3 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (5 distinct):
Recombining binding protein suppressor of hairless Chain: C
Molecule details ›
Chain: C
Length: 434 amino acids
Theoretical weight: 49.29 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q06330 (Residues: 22-449; Coverage: 86%)
Gene names: IGKJRB, IGKJRB1, RBPJ, RBPJK, RBPSUH
Sequence domains:
Structure domains:
Notch 1 intracellular domain Chain: K
Molecule details ›
Chain: K
Length: 256 amino acids
Theoretical weight: 27.89 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P46531 (Residues: 1872-2126; Coverage: 10%)
Gene names: NOTCH1, TAN1
Sequence domains:
Structure domains: Ankyrin repeat-containing domain
Mastermind-like protein 1 Chain: M
Molecule details ›
Chain: M
Length: 63 amino acids
Theoretical weight: 7.53 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q92585 (Residues: 13-74; Coverage: 6%)
Gene names: KIAA0200, MAML1
Sequence domains: MamL-1 domain
Structure domains: Single alpha-helices involved in coiled-coils or other helix-helix interfaces
5'-D(*GP*TP*TP*AP*CP*TP*GP*TP*GP*GP*GP*AP*AP*AP*GP*AP*AP*A)-3' Chain: X
Molecule details ›
Chain: X
Length: 18 nucleotides
Theoretical weight: 5.63 KDa
5'-D(*TP*TP*TP*CP*TP*TP*TP*CP*CP*CP*AP*CP*AP*GP*TP*AP*AP*C)-3' Chain: Y
Molecule details ›
Chain: Y
Length: 18 nucleotides
Theoretical weight: 5.4 KDa

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P6322
Unit cell:
a: 273.866Å b: 273.866Å c: 121.016Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.241 0.222 0.257
Expression system: Escherichia coli

Quick links

Citations

78 review citations

The canonical Notch signaling pathway: unfolding the activation mechanism.
Kopan et al. (2009)
77 more

25 mentions without citation

The molecular logic of Notch signaling--a structural and biochemical perspective.
Gordon et al. (2008)
24 more