2f8t Summary


Crystal structure of Aa-Ago with externally-bound siRNA

The structure was published by Yuan, Y.R., Pei, Y., Chen, H.Y., Tuschl, T., and Patel, D.J., in 2006 in a paper entitled "A Potential Protein-RNA Recognition Event along the RISC-Loading Pathway from the Structure of A. aeolicus Argonaute with Externally Bound siRNA." (abstract).

This crystal structure was determined using X-ray diffraction at a resolution of 3.1 Å and deposited in 2005.

The experimental data on which the structure is based was also deposited.

This PDB entry contains a complex of 2 biomacromolecules, namely 26-MER and Argonaute protein.

The molecule has more than one probable quaternary state observed. For more details see the quaternary structure page.

The following tables show cross-reference information to other databases (to obtain a list of all PDB entries sharing the same property or classification, click on the magnifying glass icon):

Chain Name UniProt Name of source organism % of UniProt sequence present in the sample Residues in the sample molecules % of residues observed
A Argonaute protein O67434 (1-706) (O67434_AQUAE)search Aquifex aeolicus VF5search 100% 706 99%
B Argonaute protein O67434 (1-706) (O67434_AQUAE)search Aquifex aeolicus VF5search 100% 706 99%

This entry contains 1 unique UniProt protein:

UniProt accession Name Organism PDB
O67434 (1 - 706) Argonaute protein Aquifex aeolicus

Chain Structural classification (SCOP) Structural classification (CATH) Sequence family (Pfam)
A, B (O67434) PAZ domainsearch, PIWI domainsearch PAZ domain superfamilysearch, Rossmann foldsearch, Nucleotidyltransferase; domain 5search PF02171: Piwi domainsearch

Chain ID Molecular function (GO)
A, B (O67434) metal ion bindingsearch nucleic acid bindingsearch

Chain InterPro annotation
A, B PAZ domainsearch Stem cell self-renewal protein Piwisearch Ribonuclease H-like domainsearch