› 2f6j

X-ray diffraction
2Å resolution

Crystal structure of PHD finger-linker-bromodomain fragment of human BPTF in the H3(1-15)K4me3 bound state

Released:
DOI: 10.2210/pdb2f6j/pdb
PDB entry 2f6j coloured by chain, front view.
PDB entry 2f6j coloured by chain, side view.
PDB entry 2f6j coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Molecular basis for site-specific read-out of histone H3K4me3 by the BPTF PHD finger of NURF.
Li H, Ilin S, Wang W, Duncan EM, Wysocka J, Allis CD, Patel DJ
Nature 442 91-5 (2006)
PMID: 16728978

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-162687 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Nucleosome-remodeling factor subunit BPTF Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 174 amino acids
Theoretical weight: 20.53 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q12830 (Residues: 2865-3033; Coverage: 6%)
Gene names: BPTF, FAC1, FALZ
Sequence domains:
Structure domains:
histone H3, N-terminal Chain: P
Molecule details ›
Chain: P
Length: 15 amino acids
Theoretical weight: 1.61 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand ZN 6 x ZN
2 modified residues:
Ligand MSE 15 x MSE
Ligand M3L 1 x M3L

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: P21
Unit cell:
a: 38.579Å b: 84.987Å c: 109.385Å
α: 90° β: 99.23° γ: 90°
R-values:
R R work R free
0.198 0.198 0.227
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

133 review citations

The epigenomics of cancer.
Jones et al. (2007)
132 more

37 mentions without citation

How chromatin-binding modules interpret histone modifications: lessons from professional pocket pickers.
Taverna et al. (2007)
36 more