2f49 Summary


Crystal structure of Fus3 in complex with a Ste5 peptide

The structure was published by Bhattacharyya, R.P., Remenyi, A., Good, M.C., Bashor, C.J., Falick, A.M., and Lim, W.A., in 2006 in a paper entitled "The Ste5 scaffold allosterically modulates signaling output of the yeast mating pathway" (abstract).

This crystal structure was determined using X-ray diffraction at a resolution of 1.9 Å and deposited in 2005.

The experimental data on which the structure is based was also deposited.

This PDB entry contains a complex of 2 biomacromolecules, namely Mitogen-activated protein kinase FUS3 and STE5 peptide.

It also contains one or more heterogenic compounds (e.g., ligands, co-factors, ions, modified amino acids, etc.); see here for a complete list.

The molecule has more than one probable quaternary state observed. For more details see the quaternary structure page.

The following tables show cross-reference information to other databases (to obtain a list of all PDB entries sharing the same property or classification, click on the magnifying glass icon):

Chain Name UniProt Name of source organism % of UniProt sequence present in the sample Residues in the sample molecules % of residues observed
A Mitogen-activated protein kinase FUS3 P16892 (1-353) (FUS3_YEAST)search Saccharomyces cerevisiae S288csearch 100% 353 95%
B Mitogen-activated protein kinase FUS3 P16892 (1-353) (FUS3_YEAST)search Saccharomyces cerevisiae S288csearch 100% 353 95%
C STE5 peptide Not available
Synthetic Not available 30 63%

This entry contains 1 unique UniProt protein:

UniProt accession Name Organism PDB
P16892 (1 - 353) Mitogen-activated protein kinase FUS3 Saccharomyces cerevisiae

Chain Structural classification (CATH) Sequence family (Pfam)
A, B (P16892) Phosphorylase Kinase; domain 1search, Transferase(Phosphotransferase) domain 1search PF00069: Protein kinase domainsearch

Chain ID Biological process (GO) Molecular function (GO) Cellular component (GO)
A, B (P16892) MAPK cascadesearch negative regulation of MAPK cascadesearch cell cycle arrestsearch invasive growth in response to glucose limitationsearch positive regulation of protein export from nucleussearch protein phosphorylationsearch mitotic nuclear divisionsearch pheromone-dependent signal transduction involved in conjugation with cellular fusionsearch negative regulation of transposition, RNA-mediatedsearch cell cyclesearch conjugationsearch phosphorylationsearch cell divisionsearch protein bindingsearch protein kinase activitysearch transferase activity, transferring phosphorus-containing groupssearch MAP kinase activitysearch ATP bindingsearch protein serine/threonine kinase activitysearch identical protein bindingsearch nucleotide bindingsearch transferase activitysearch kinase activitysearch nucleussearch cytoplasmsearch mating projection tipsearch periplasmic spacesearch

Chain InterPro annotation
A, B Protein kinase domainsearch Serine/threonine/dual specificity protein kinase, catalytic domainsearch Mitogen-activated protein (MAP) kinase, conserved sitesearch Serine/threonine-protein kinase, active sitesearch Protein kinase-like domainsearch Protein kinase, ATP binding sitesearch
C Scaffold protein Ste5, Fus5-binding domainsearch