› 2f49

X-ray diffraction
1.9Å resolution

Crystal structure of Fus3 in complex with a Ste5 peptide

Released:
DOI: 10.2210/pdb2f49/pdb
PDB entry 2f49 coloured by chain, front view.
PDB entry 2f49 coloured by chain, side view.
PDB entry 2f49 coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
The Ste5 scaffold allosterically modulates signaling output of the yeast mating pathway.
Bhattacharyya RP, Reményi A, Good MC, Bashor CJ, Falick AM, Lim WA
Science 311 822-6 (2006)
PMID: 16424299

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero dimer (preferred)
monomeric
hetero trimer
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-147837 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Mitogen-activated protein kinase FUS3 Chains: A, B
Molecule details ›
Chains: A, B
Length: 353 amino acids
Theoretical weight: 40.81 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P16892 (Residues: 1-353; Coverage: 100%)
Gene names: DAC2, FUS3, YBL016W, YBL03.21, YBL0303
Sequence domains: Protein kinase domain
Structure domains:
STE5 peptide Chain: C
Molecule details ›
Chain: C
Length: 30 amino acids
Theoretical weight: 3.35 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand MG 2 x MG
Ligand SCN 7 x SCN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P212121
Unit cell:
a: 94.622Å b: 95.21Å c: 101.662Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.201 0.201 0.234
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

47 review citations

Mechanisms of specificity in protein phosphorylation.
Ubersax et al. (2007)
46 more

7 mentions without citation

Scaffold proteins: hubs for controlling the flow of cellular information.
Good et al. (2011)
6 more