2ez6 Summary

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Crystal structure of Aquifex aeolicus RNase III (D44N) complexed with product of double-stranded RNA processing

The structure was published by Gan, J., Tropea, J.E., Austin, B.P., Court, D.L., Waugh, D.S., and Ji, X., in 2006 in a paper entitled "Structural Insight into the Mechanism of Double-Stranded RNA Processing by Ribonuclease III." (abstract).

This crystal structure was determined using X-ray diffraction at a resolution of 2.05 Å and deposited in 2005.

The experimental data on which the structure is based was also deposited.

This PDB entry contains a complex of 2 biomacromolecules, namely 28-MER and Ribonuclease III.

It also contains one or more heterogenic compounds (e.g., ligands, co-factors, ions, modified amino acids, etc.); see here for a complete list.

The molecule most likely forms heterotetramers.

The following tables show cross-reference information to other databases (to obtain a list of all PDB entries sharing the same property or classification, click on the magnifying glass icon):


Chain Name UniProt Name of source organism % of UniProt sequence present in the sample Residues in the sample molecules % of residues observed
A Ribonuclease III O67082 (1-221) (RNC_AQUAE)search Aquifex aeolicus VF5search 100% 221 98%
B Ribonuclease III O67082 (1-221) (RNC_AQUAE)search Aquifex aeolicus VF5search 100% 221 98%


This entry contains 1 unique UniProt protein:

UniProt accession Name Organism PDB
O67082 (1 - 221) Ribonuclease III Aquifex aeolicus

Chain Structural classification (SCOP) Structural classification (CATH) Sequence family (Pfam)
A, B (O67082) RNase III catalytic domain-likesearch, Double-stranded RNA-binding domain (dsRBD)search Ribonuclease iii, N-terminal Endonuclease Domain; Chain Asearch, Double Stranded RNA Binding Domainsearch PF00035: Double-stranded RNA binding motifsearch, PF00636: Ribonuclease III domainsearch

Chain ID Molecular function (GO) Cellular component (GO) Biological process (GO)
A, B (O67082) RNA bindingsearch nuclease activitysearch endonuclease activitysearch ribonuclease III activitysearch hydrolase activitysearch metal ion bindingsearch cytoplasmsearch rRNA processingsearch RNA processingsearch mRNA processingsearch tRNA processingsearch rRNA catabolic processsearch nucleic acid phosphodiester bond hydrolysissearch RNA phosphodiester bond hydrolysissearch RNA phosphodiester bond hydrolysis, endonucleolyticsearch

Chain InterPro annotation
A, B Ribonuclease III domainsearch Ribonuclease IIIsearch Double-stranded RNA-binding domainsearch