› 2exi

X-ray diffraction
2.15Å resolution

Structure of the family43 beta-Xylosidase D15G mutant from geobacillus stearothermophilus

Released:
DOI: 10.2210/pdb2exi/pdb
PDB entry 2exi coloured by chain, front view.
PDB entry 2exi coloured by chain, side view.
PDB entry 2exi coloured by chain, top view.
Source organism: Geobacillus stearothermophilus
Primary publication:
The structure of an inverting GH43 beta-xylosidase from Geobacillus stearothermophilus with its substrate reveals the role of the three catalytic residues.
Brüx C, Ben-David A, Shallom-Shezifi D, Leon M, Niefind K, Shoham G, Shoham Y, Schomburg D
J Mol Biol 359 97-109 (2006)
PMID: 16631196

Function and Biology Details

Reaction catalysed: 
Hydrolysis of (1->4)-beta-D-xylans, to remove successive D-xylose residues from the non-reducing termini
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-170833 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Beta-xylosidase C-terminal Concanavalin A-like domain-containing protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 535 amino acids
Theoretical weight: 61.93 KDa
Source organism: Geobacillus stearothermophilus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q09LX0 (Residues: 1-535; Coverage: 100%)
Gene name: xynB3
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand CA 4 x CA
Ligand MES 3 x MES
Ligand GOL 4 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X12
Spacegroup: P43212
Unit cell:
a: 140.175Å b: 140.175Å c: 232.322Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.203 0.199 0.276
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

β-xylosidases and α-L-arabinofuranosidases: accessory enzymes for arabinoxylan degradation.
Lagaert et al. (2014)
3 more

2 mentions without citation

Functional metagenomics unveils a multifunctional glycosyl hydrolase from the family 43 catalysing the breakdown of plant polymers in the calf rumen.
Ferrer et al. (2012)
1 more