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X-ray diffraction
2.4Å resolution

Crystal Structure of the (S)-Specific 1-Phenylethanol Dehydrogenase of the Denitrifying Bacterium Strain EbN1

Released:
DOI: 10.2210/pdb2ewm/pdb
PDB entry 2ewm coloured by chain, front view.
PDB entry 2ewm coloured by chain, side view.
PDB entry 2ewm coloured by chain, top view.
Source organism: Azoarcus
Primary publication:
Crystal structure and enzyme kinetics of the (S)-specific 1-phenylethanol dehydrogenase of the denitrifying bacterium strain EbN1.
Höffken HW, Duong M, Friedrich T, Breuer M, Hauer B, Reinhardt R, Rabus R, Heider J
Biochemistry 45 82-93 (2006)
PMID: 16388583

Function and Biology Details

Reaction catalysed: 
(S)-1-phenylethanol + NAD(+) = acetophenone + NADH
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-177742 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
(S)-1-Phenylethanol dehydrogenase Chains: A, B
Molecule details ›
Chains: A, B
Length: 249 amino acids
Theoretical weight: 26.69 KDa
Source organism: Azoarcus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5P5I4 (Residues: 1-249; Coverage: 100%)
Gene names: AZOSEA13030, c1A58, ped
Sequence domains: Enoyl-(Acyl carrier protein) reductase
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments


Cofactor: Ligand NAD 2 x NAD
1 bound ligand:
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P43212
Unit cell:
a: 58.4Å b: 58.4Å c: 236.2Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.176 0.176 0.256
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Microbial degradation of aromatic compounds - from one strategy to four.
Fuchs et al. (2011)
5 more

5 mentions without citation

Conversion of alcohols to enantiopure amines through dual-enzyme hydrogen-borrowing cascades.
Mutti et al. (2015)
4 more