› 2ewh

X-ray diffraction
1.4Å resolution

Carboxysome protein CsoS1A from Halothiobacillus neapolitanus

Released:
DOI: 10.2210/pdb2ewh/pdb
PDB entry 2ewh coloured by chain, front view.
PDB entry 2ewh coloured by chain, side view.
PDB entry 2ewh coloured by chain, top view.
Source organism: Halothiobacillus neapolitanus
Primary publication:
Structural analysis of CsoS1A and the protein shell of the Halothiobacillus neapolitanus carboxysome.
Tsai Y, Sawaya MR, Cannon GC, Cai F, Williams EB, Heinhorst S, Kerfeld CA, Yeates TO
PLoS Biol 5 e144 (2007)
PMID: 17518518

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155328 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Major carboxysome shell protein CsoS1A Chain: A
Molecule details ›
Chain: A
Length: 98 amino acids
Theoretical weight: 9.97 KDa
Source organism: Halothiobacillus neapolitanus
Expression system: Escherichia coli
UniProt:
  • Canonical: P45689 (Residues: 1-98; Coverage: 100%)
Gene names: Hneap_0915, csoS1, csoS1A
Sequence domains: BMC domain
Structure domains: Alpha-Beta Plaits

Ligands and Environments

2 bound ligands:
Ligand TRS 1 x TRS
Ligand EDO 1 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.2
Spacegroup: P6
Unit cell:
a: 66.417Å b: 66.417Å c: 28.982Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.187 0.185 0.24
Expression system: Escherichia coli

Quick links

Citations

21 review citations

Frontiers, opportunities, and challenges in biochemical and chemical catalysis of CO2 fixation.
Appel et al. (2013)
20 more

9 mentions without citation

Recent structural insights into bacterial microcompartment shells.
Ochoa et al. (2021)
8 more