› 2ewc

X-ray diffraction
2.15Å resolution

Structure of hypothetical protein from Streptococcus pyogenes M1 GAS, member of highly conserved yjgF family of proteins

Released:
DOI: 10.2210/pdb2ewc/pdb
PDB entry 2ewc coloured by chain, front view.
PDB entry 2ewc coloured by chain, side view.
PDB entry 2ewc coloured by chain, top view.
Source organism: Streptococcus pyogenes M1 GAS
Entry authors: Nocek B, Li H, Clancy S, Collart F, Joachimiak A, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:
Structure domain:

Structure analysis Details

Assemblies composition:
homo dodecamer
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-189407 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Translation initiation inhibitor Chains: A, B, C, D, E, F, G, H, I, J, K, L
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L
Length: 126 amino acids
Theoretical weight: 14.7 KDa
Source organism: Streptococcus pyogenes M1 GAS
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q99XS4 (Residues: 1-126; Coverage: 100%)
Gene name: SPy_2060
Structure domains: RutC-like

Ligands and Environments

1 bound ligand:
Ligand GOL 5 x GOL
1 modified residue:
Ligand MSE 72 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P212121
Unit cell:
a: 105.12Å b: 131.994Å c: 135.64Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.164 0.162 0.204
Expression system: Escherichia coli BL21(DE3)

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